Post-Hercules work
After Hercules ended, Sorbo played the starring role of Captain Dylan Hunt in the science-fiction drama series Andromeda from 2000 to 2005. In 2006, he played a recurring role on the final season of The O.C.
In 2007, he starred in two direct-to-video sequels to Walking Tall, Walking Tall: The Payback and Walking Tall: Lone Justice. In the Hallmark Channel film Avenging Angel, he co-starred with his wife, Sam Jenkins.
In 2008, he appeared in the spoof film titled Meet the Spartans.
In the first of what would be several voice acting jobs, Sorbo performed one of the main protagonists, Prometheus, in the video game The Conduit. He reprised the role of Hercules in the video game God of War III and worked together with his wife on the game Cloudberry Kingdom.
In 2014, Sorbo co-starred in God's Not Dead, a Christian film in which he portrayed an atheist college professor who requires his students to disown their religions on the first day of his class.
Although heavily panned by critics, it was a commercial success and the first of a series of Christian films that Sorbo would produce or perform.
Sorbo appeared in the 2015 romantic comedy Single in South Beach, starring Haley Webb. On Twitter, in 2019, she accused Sorbo of sexual advances and of sexually harassing her on the set. However, Webb did not elaborate on any of her accusations, and he did not respond to her tweet.
In 2017, Sorbo directed and starred in the Christian drama film Let There Be Light, which featured conservative pundit Sean Hannity, who was also an executive producer. It was described by The New Yorker as "a cynical, xenophobic morality tale, as bitter as it is saccharine" that closes with "a dénouement of bald Islamophobia". The Los Angeles Times, although critical, says that it provides "an involving bonanza" for its intended Christian audience. Sorbo is described as bringing "puckish charm and credible warmth to his role".
That same year, Sorbo guest-starred in three episodes of the superhero television series Supergirl. Sorbo portrayed Lar Gand with Teri Hatcher as his wife Rhea. Coincidentally, Sorbo was almost cast as Superman opposite Hatcher's Lois Lane 24 years earlier in Lois and Clark: The New Adventures of Superman.
In 2024, he appeared in the Ronald Reagan biopic Reagan opposite Dennis Quaid, who plays the title role.